Simple method for the isolation of encephalomyocarditis virus ribonucleic acid.
نویسندگان
چکیده
A simple purification procedure for encephalomyocarditis virus is described, yielding material suitable for extraction of viral ribonucleic acid for use as messenger in animal cell-free systems.
منابع مشابه
Encephalomyocarditis virus ribonucleic acid polymerase associated with 150S cytoplasmic particles.
Cytoplasmic particles which sedimented at 150S were the smallest structures containing detectable viral ribonucleic acid polymerase in mouse cells infected with encephalomyocarditis virus.
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Martin, Malec, Sved & Work (1961 b) showed that, during a single growth cycle, infection with encephalomyocarditis virus caused no changes in the total deoxyribonucleic acid, ribonucleic acid or protein of the host ascites-tumour cell. However, by the use of [6-14C]orotic acid and [14C]valine, it was shown that there were substantial changes in the rates of turnover of ribonucleic acid andprote...
متن کاملCharacterization of the polypeptides formed in response to encephalomyocarditis virus ribonucleic acid in a cell-free system from mouse ascites tumor cells.
The polypeptide products synthesized at different times in a cell-free system from Krebs mouse ascites tumor cells in response to the addition of encephalomyocarditis (EMC) virus ribonucleic acid (RNA) were characterized by electrophoresis on polyacrylamide gels and fingerprint analysis of their tryptic peptides. Translation of the EMC RNA genome with time occurred in a nonrandom fashion in the...
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An emulsifier which had an environmental relationship to Reye's syndrome, when used to treated L-929 cultures, was shown to increase the rate of encephalomyocarditis virus penetration and uncoating while having no effect on the attachment of virus or on the replication of infectious ribonucleic acid. This treatment also rendered L-929 cells unable to respond normally to interferon inducers and ...
متن کاملMethod for increasing the regularity of isolation of infectious ribonucleic acid from influenza virus.
A more effective method of isolating infectious ribonucleic acid (RNA) from influenza virus was evaluated based on the enzymatic disintegration of the viral coat by Pronase, followed by phenol-detergent extraction of the RNA from susceptible and from resistant cells.
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عنوان ژورنال:
- Journal of virology
دوره 9 3 شماره
صفحات -
تاریخ انتشار 1972